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KMID : 0378019860290020113
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New Medical Journal
1986 Volume.29 No. 2 p.113 ~ p.124
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Effect of Prednisolone Injection the Intestinal Pathology of Mice Experimentally Infected with Metagonimus yokogawi
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Abstract
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An experimental study on Metagonimus yokogawai infection was performed to know the effect of prednisolone, an immunosuppressive agent, on the intestinal pathology of mice and extent of worm invasion into their intestinal wall. The metacercariae of M. yokogawi i were obtained from the flesh of sweetfish, Plecoglossus altivelis, by peptic digestion technique. A total of 54 male mice, ICR strain, were divided into three groups; 6 normal contra Is, 24 fluke infection group, and 24 fluke infection and prednisolone injection group. T e latter two groups were fed with 1, 800 metacercariae per each mouse and sacrificed seguentially at 6 hours, 12 hours, I day, 3 days, 5 days, i week, 2 weeks and 3 weeks after t e infection. Prednisolone injection to the last group was done every other day from 7 da s before infection until sacrifice, with the dose of 10mg/kg of body weight each time into the subcutaneous tissue of inner thigh. Immediately after sacrifice of mice, their duodenum , jejunum and ileum were resected, fixed, cut into thin sections, stained with hematoxyli and eosin, and observed.
The results are as follows:
1. In normal controls, the segments of small intestine revealed wellpreserved mucos.1 layer which is composed of villi and crypt, submucosa and deeper layers, except for sligh degrees of non-specific inflammatory reactions in the stroma of villi.
2. In fluke infection group, many sectioned worms were found until 1 day after infectio, and pathological findings were observed only in mucosal layer, especially in villi. The villous pathology was characterized by edema at tip portions, pressure atrophy in epithelial layers adjacent to worms, and inflammatory cell infiltration in the stroma. However, afte 3 days from infection, nearly no worms were found in intestinal sections and, after 1 week, the intestinal pathology became minimal in extent.
3. In prednisolone-injected mice, the flukes were consistently found sectioned throughout whole experimental period, which suggests successful parasitism of M yokogawai. There was nearly no tissue reaction in the villous stroma through whole experimental period. Intestinal pathology was also minimal until 3 days after infection, however, after 5 days, there was marked destruction of mucosal layers in the duodenum and jejunum, and con piete obliteration of mucosal integrity was sometimes observed. At this time the flukes were found to have invaded deeply into the submucosa facing the muscular layer of intestinal wall. But the mucosal disruption was gradually recovered after 1 week of infection.
From the results, it is concluded that immunosuppression by prednisoione injection an induce severe mucosal damage in small intestine of mice experimentally infected with M yokogawai, although it is not certain whether such damage is directly due to invasion of worm itself,or due to other reason such as activation of bacterial or viral infection.
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